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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-34890 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMYD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, DDDDK-tagged SMYD3-transfected 293T. Predicted reactivity: Human (99%), Mouse (93%), Rat (95%), Pig (95%), Rhesus Monkey (99%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SMYD3 (Product # PA5-34890) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HepG2 chromatin extract and 5 µg of either control rabbit IgG or SMYD3 Polyclonal Antibody (Product # PA5-34890). The precipitated DNA was detected by PCR with primer set targeting to 5 flanking region of Nkx2.8.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMYD3 Polyclonal Antibody immunoprecipitates SMYD3 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 2.5 µg of pre-immune rabbit IgG B : Immunoprecipitation of SMYD3 by 2.5 µg of SMYD3 Polyclonal Antibody (Product # PA5-34890) 10% SDS-PAGE The immunoprecipitated SMYD3 protein was detected by SMYD3 Polyclonal Antibody (Product # PA5-34890) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
