Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- V3421 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- S100B Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific S100B antibody is suitable for use in Immunofluorescence/Western blot/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4C4.9
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the S100B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples using S100B antibody (clone 4C4.9). Predicted molecular weight: 12-15 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human brain tissue lysate using S100B antibody (clone 4C4.9). Predicted molecular weight: 12-15 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence testing of A2058 cells and Alexa Fluor 488 labeled S100B antibody (clone 4C4.9).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence testing of A2058 cells and Alexa Fluor 488 labeled S100B antibody (clone 4C4.9).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human melanoma stained with S100B antibody + AEC chromogen (clone 4C4.9). No special pretreatment is required for IHC staining of formalin-paraffin tissues.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free S100B antibody (clone 4C4.9) as confirmation of integrity and purity.