Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [3]
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Validation data
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- Product number
- Ab008408 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- S100B Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human S100B Antibody, Monoclonal (5H2-3), is a Detection or Capture antibody could be used for ELISA, CLIA, LF, GICA, FIA.CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100µg/4µg AEFLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 5H2-3
- Vial size
- 100_g,10_g,1mg,50_g
- Concentration
- Lot by Lot
- Storage
- Store at 37¡C short term (7 days). Store at 4¡C short term (30 days). Store at -20±5¡C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Sandwich ELISA - S100B protein standard curve S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008408) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Sensitivity: 30.3 pg/mL Range: 0.1 ng/mL - 3.2 ng/mL
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Sandwich ELISA - S100B protein standard curve S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008407) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Sensitivity: 18.8 pg/mL Range: 0.13 ng/mL - 3.1 ng/mL
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA ·Capture antibody: S100B Mouse mAb (Ab008408) ·Detection antibody: S100B Mouse mAb (Ab008407)