Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1820 - Provider product page
- Provider
- R&D Systems
- Product name
- Human S100B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human S100B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) S100A1, rhS100A4, and rhS100A6 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P04271
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Disease Progression-Dependent Effects of TREM2 Deficiency in a Mouse Model of Alzheimer's Disease.
Increased serum S100B levels in chronic schizophrenic patients on long-term clozapine or typical antipsychotics.
Jay TR, Hirsch AM, Broihier ML, Miller CM, Neilson LE, Ransohoff RM, Lamb BT, Landreth GE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jan 18;37(3):637-647
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jan 18;37(3):637-647
Increased serum S100B levels in chronic schizophrenic patients on long-term clozapine or typical antipsychotics.
Qi LY, Xiu MH, Chen DC, Wang F, Kosten TA, Kosten TR, Zhang XY
Neuroscience letters 2009 Sep 22;462(2):113-7
Neuroscience letters 2009 Sep 22;462(2):113-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human S100B by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for S100B at approximately 4 kDa (as indicated) using 10 µg/mL of Goat Anti-Human S100B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1820) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- S100B in Human Alzheimer'S Brain. S100B was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human S100B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.