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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PPS057 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal NMDA R, NR2B Subunit Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, and rat ~180 kDa NMDA Receptor NR2B subunit phosphorylated at Y1336 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage, = -20degrees C is recommended. Product is stable at = -20degrees C for at least 1 year.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detects Phospho-GRIN2B/NMDAR2B (Y1336) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1336 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l PPase). The blot is identical to the control except that it was incubated in l PPase (1200 units for 30 minutes) before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1336). The immunolabeling is completely eliminated by treatment with l PPase.
