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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PPS014 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Phospho-GRIN2B/NMDAR2B (Y1472) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, rat ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Y1472
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
Submitted references Interleukin-1-induced interleukin-6 synthesis is mediated by the neutral sphingomyelinase/Src kinase pathway in neurones.
Tsakiri N, Kimber I, Rothwell NJ, Pinteaux E
British journal of pharmacology 2008 Feb;153(4):775-83
British journal of pharmacology 2008 Feb;153(4):775-83
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Phospho-GRIN2B/NMDAR2B (Y1472) by Western Blot Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1472). The immunolabeling is completely eliminated by treatment with lambda-PPase.
