Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PPS056 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat/Primate Phospho-GRIN2B/NMDAR2B (Y1252) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, non-human primates, and rat ~180 kDa NMDA Receptor NR2B subunit phosphorylated at Y1252 in Western blots.
- Reactivity
- Human, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
Submitted references Early failure of N-methyl-D-aspartate receptors and deficient spine formation induced by reduction of regulatory heme in neurons.
Chernova T, Steinert JR, Richards P, Mistry R, Challiss RA, Jukes-Jones R, Cain K, Smith AG, Forsythe ID
Molecular pharmacology 2011 May;79(5):844-54
Molecular pharmacology 2011 May;79(5):844-54
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of GRIN2B/NMDAR2B (Y1252) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1252 (Control). The phosphospecificity of this labeling is shown in the second lane ( lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1252). The immunolabeling is completely eliminated by treatment with lambda PPase.