PPS056

antibody from R&D Systems

Targeting: GRIN1 GluN1, NMDAR1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PPS056

Provider

R&D Systems

Product name

Human/Rat/Primate Phospho-GRIN2B/NMDAR2B (Y1252) Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Human, non-human primates, and rat ~180 kDa NMDA Receptor NR2B subunit phosphorylated at Y1252 in Western blots.

Reactivity

Human, Rat, Simian

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 uL

Storage

For long-term storage,

References

Submitted references

Early failure of N-methyl-D-aspartate receptors and deficient spine formation induced by reduction of regulatory heme in neurons.

Chernova T, Steinert JR, Richards P, Mistry R, Challiss RA, Jukes-Jones R, Cain K, Smith AG, Forsythe ID
Molecular pharmacology 2011 May;79(5):844-54

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of GRIN2B/NMDAR2B (Y1252) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDAR phosphorylated at Y1252 (Control). The phosphospecificity of this labeling is shown in the second lane ( lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1252). The immunolabeling is completely eliminated by treatment with lambda PPase.