A010-31

antibody from Badrilla Ltd

Targeting: RYR2 ARVC2, ARVD2, VTSIP

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

A010-31

Provider

Badrilla Ltd

Proper citation

Badrilla Cat#A010-31, RRID:AB_2617055

Product name

RYR2 Phospho Ser-2814 Anti-Serum

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The ryanodine receptor (RyR2) is a Ca2+ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca2+ to RyR2 opens the channel and Ca2+ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. CaMKII was able to phosphorylate Ser-2814 of RYR2 enhancing Ca2+- sensitivity and increasing open probability. Excessive phosphorylation of Ser-2814 leads to atrial fibrillation, arrhythmias and sudden death.

Host

Rabbit

Antigen sequence

TSQVS(PO3H2)VDAAH2819


Isotype

IgG

Vial size

50 µl

Storage

Lyophilised: 4 °C Reconstituted:-80 °C

Handling

Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles

References

Submitted references

JTV519 (K201) reduces sarcoplasmic reticulum Ca²⁺ leak and improves diastolic function in vitro in murine and human non-failing myocardium.

Sacherer M, Sedej S, Wakuła P, Wallner M, Vos MA, Kockskämper J, Stiegler P, Sereinigg M, von Lewinski D, Antoons G, Pieske BM, Heinzel FR, CONTICA investigators
British journal of pharmacology 2012 Oct;167(3):493-504

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Mutation of the calmodulin binding motif IQ of the L-type Ca(v)1.2 Ca2+ channel to EQ induces dilated cardiomyopathy and death.

Blaich A, Pahlavan S, Tian Q, Oberhofer M, Poomvanicha M, Lenhardt P, Domes K, Wegener JW, Moosmang S, Ruppenthal S, Scholz A, Lipp P, Hofmann F
The Journal of biological chemistry 2012 Jun 29;287(27):22616-25

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Caveolae compartmentalise β2-adrenoceptor signals by curtailing cAMP production and maintaining phosphatase activity in the sarcoplasmic reticulum of the adult ventricular myocyte.

Macdougall DA, Agarwal SR, Stopford EA, Chu H, Collins JA, Longster AL, Colyer J, Harvey RD, Calaghan S
Journal of molecular and cellular cardiology 2012 Feb;52(2):388-400

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Age-related regulation of excitation-contraction coupling in rat heart.

Kandilci HB, Tuncay E, Zeydanli EN, Sozmen NN, Turan B
Journal of physiology and biochemistry 2011 Sep;67(3):317-30

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Nav1.5-dependent persistent Na+ influx activates CaMKII in rat ventricular myocytes and N1325S mice.

Yao L, Fan P, Jiang Z, Viatchenko-Karpinski S, Wu Y, Kornyeyev D, Hirakawa R, Budas GR, Rajamani S, Shryock JC, Belardinelli L
American journal of physiology. Cell physiology 2011 Sep;301(3):C577-86

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Disrupted junctional membrane complexes and hyperactive ryanodine receptors after acute junctophilin knockdown in mice.

van Oort RJ, Garbino A, Wang W, Dixit SS, Landstrom AP, Gaur N, De Almeida AC, Skapura DG, Rudy Y, Burns AR, Ackerman MJ, Wehrens XH
Circulation 2011 Mar 8;123(9):979-88

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Conditional FKBP12.6 overexpression in mouse cardiac myocytes prevents triggered ventricular tachycardia through specific alterations in excitation-contraction coupling.

Gellen B, Fernández-Velasco M, Briec F, Vinet L, LeQuang K, Rouet-Benzineb P, Bénitah JP, Pezet M, Palais G, Pellegrin N, Zhang A, Perrier R, Escoubet B, Marniquet X, Richard S, Jaisser F, Gómez AM, Charpentier F, Mercadier JJ
Circulation 2008 Apr 8;117(14):1778-86