Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A010-32 - Provider product page
- Provider
- Badrilla Ltd
- Product name
- Anti-RYR2 Phospho Serine-2030 IgG
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The ryanodine receptor (RyR2) is a Ca2+ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca2+ to RyR2 opens the channel and Ca2+ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. PKA, and not CaMKII, was able to phosphorylate Ser-2030 of RYR2 in vitro and in isolated cardiac myocyte cells.
- Reactivity
- Mouse, Rat, Canine, Rabbit
- Host
- Rabbit
- Antigen sequence
TIRGRLLS(PO3H2)LVE203
- Isotype
- IgG
- Vial size
- 50 µl
- Storage
- Lyophilised: 4 °C Reconstituted:-80 °C
- Handling
- Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles
Submitted references Caveolae compartmentalise β2-adrenoceptor signals by curtailing cAMP production and maintaining phosphatase activity in the sarcoplasmic reticulum of the adult ventricular myocyte.
Macdougall DA, Agarwal SR, Stopford EA, Chu H, Collins JA, Longster AL, Colyer J, Harvey RD, Calaghan S
Journal of molecular and cellular cardiology 2012 Feb;52(2):388-400
Journal of molecular and cellular cardiology 2012 Feb;52(2):388-400
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Supportive validation
- Submitted by
- Badrilla Ltd (provider)
- Main image
- Experimental details
- Western blot
- Sample type
- HEK cell lysate expressing recombinant RyR2 incubated with PKA +/- ATP
- Validation comment
- Antibody stains for RyR2 when phosphorylated by PKA
- Primary Ab dilution
- 3.513888889
- Secondary Ab dilution
- 1.430555556
- Protocol
- Protocol