17146-1-AP

antibody from Proteintech Group

Targeting: PYCR2 P5CR2

Provider product page for 17146-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [22]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 17146-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17146-1-AP, RRID:AB_2253344
Product name: PYCR2 antibody
Antibody type: Polyclonal
Description: KD/KO validated PYCR2 antibody (Cat. #17146-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PYCR2 protein structure - 17146-1-AP shown in red.

Submitted references Exploration of influenza A virus PA protein-associated cellular proteins discloses its impact on mitochondrial function.

Wu CC, Tam EH, Shih YY, Lin YR, Hsueh PC, Shen HY, Woung CH, Wang LT, Tsai JC, Lin SJ, Chang CR, Ke PY, Kuo RL
Virus research 2024 Jul;345:199387

PYCR2, induced by c-Myc, promotes the invasiveness and metastasis of breast cancer by activating AKT signalling pathway.

Wu G, Qin S, Gu K, Zhou Y
The international journal of biochemistry & cell biology 2024 Jan;166:106506

ALKBH5-PYCR2 Positive Feedback Loop Promotes Proneural-Mesenchymal Transition Via Proline Synthesis In GBM.

Li L, Yang M, Pu X, Tang Y, Fei F, Li Z, Hou H, Chen Q, Wang Q, Wu Y, Zhang Y, Ren C, Gong A
Journal of Cancer 2023;14(9):1579-1591

Loss of attachment promotes proline accumulation and excretion in cancer cells.

Pilley SE, Hennequart M, Vandekeere A, Blagih J, Legrave NM, Fendt SM, Vousden KH, Labuschagne CF
Science advances 2023 Sep 8;9(36):eadh2023

PYCR2 promotes growth and aerobic glycolysis in human liver cancer by regulating the AKT signaling pathway.

Wang S, Yi W, Xu Z, Shi M
Biochemical and biophysical research communications 2023 Nov 5;680:15-24

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951

Differential Degradation of TRA2A and PYCR2 Mediated by Ubiquitin E3 Ligase E4B.

Lu Y, Jiang B, Peng K, Li S, Liu X, Wang B, Chen Y, Wang T, Zhao B
Frontiers in cell and developmental biology 2022;10:833396

Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Kay EJ, Paterson K, Riera-Domingo C, Sumpton D, Däbritz JHM, Tardito S, Boldrini C, Hernandez-Fernaud JR, Athineos D, Dhayade S, Stepanova E, Gjerga E, Neilson LJ, Lilla S, Hedley A, Koulouras G, McGregor G, Jamieson C, Johnson RM, Park M, Kirschner K, Miller C, Kamphorst JJ, Loayza-Puch F, Saez-Rodriguez J, Mazzone M, Blyth K, Zagnoni M, Zanivan S
Nature metabolism 2022 Jun;4(6):693-710

Proline synthesis through PYCR1 is required to support cancer cell proliferation and survival in oxygen-limiting conditions.

Westbrook RL, Bridges E, Roberts J, Escribano-Gonzalez C, Eales KL, Vettore LA, Walker PD, Vera-Siguenza E, Rana H, Cuozzo F, Eskla KL, Vellama H, Shaaban A, Nixon C, Luuk H, Lavery GG, Hodson DJ, Harris AL, Tennant DA
Cell reports 2022 Feb 1;38(5):110320

Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.

Chen Z, Wang C, Feng X, Nie L, Tang M, Zhang H, Xiong Y, Swisher SK, Srivastava M, Chen J
The EMBO journal 2021 Sep 1;40(17):e107776

The upregulation of PYCR2 is associated with aggressive colon cancer progression and a poor prognosis.

Wang S, Gu L, Huang L, Fang J, Liu Z, Xu Q
Biochemical and biophysical research communications 2021 Oct 1;572:20-26

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Jones B, Balasubramaniam M, Lebowitz JJ, Taylor A, Villalta F, Khoshbouei H, Grueter C, Grueter B, Dash C, Pandhare J
Scientific reports 2021 Jan 14;11(1):1422

PINCH-1 promotes Δ(1)-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Cui C, Wang J, Guo L, Wu C
Amino acids 2021 Dec;53(12):1875-1890

African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.

Chen S, Zhang X, Nie Y, Li H, Chen W, Lin W, Chen F, Xie Q
Virologica Sinica 2021 Apr;36(2):196-206

Mitochondrial NADP(+) is essential for proline biosynthesis during cell growth.

Tran DH, Kesavan R, Rion H, Soflaee MH, Solmonson A, Bezwada D, Vu HS, Cai F, Phillips JA 3rd, DeBerardinis RJ, Hoxhaj G
Nature metabolism 2021 Apr;3(4):571-585

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Schwörer S, Berisa M, Violante S, Qin W, Zhu J, Hendrickson RC, Cross JR, Thompson CB
The EMBO journal 2020 Apr 15;39(8):e103334

Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.

Gao Q, Zhu H, Dong L, Shi W, Chen R, Song Z, Huang C, Li J, Dong X, Zhou Y, Liu Q, Ma L, Wang X, Zhou J, Liu Y, Boja E, Robles AI, Ma W, Wang P, Li Y, Ding L, Wen B, Zhang B, Rodriguez H, Gao D, Zhou H, Fan J
Cell 2019 Oct 3;179(2):561-577.e22

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Guo L, Cui C, Zhang K, Wang J, Wang Y, Lu Y, Chen K, Yuan J, Xiao G, Tang B, Sun Y, Wu C
Nature communications 2019 Feb 19;10(1):845

Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.

Hollinshead KER, Munford H, Eales KL, Bardella C, Li C, Escribano-Gonzalez C, Thakker A, Nonnenmacher Y, Kluckova K, Jeeves M, Murren R, Cuozzo F, Ye D, Laurenti G, Zhu W, Hiller K, Hodson DJ, Hua W, Tomlinson IP, Ludwig C, Mao Y, Tennant DA
Cell reports 2018 Mar 20;22(12):3107-3114

A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder.

Huang YW, Chiang MF, Ho CS, Hung PL, Hsu MH, Lee TH, Chu LJ, Liu H, Tang P, Victor Ng W, Lin DS
Aging and disease 2018 Dec;9(6):1043-1057

Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.

Liu W, Hancock CN, Fischer JW, Harman M, Phang JM
Scientific reports 2015 Nov 24;5:17206

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP(PYCR2 antibody) at dilution of 1:800
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using PYCR2 antibody 17146-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human ovary using 17146-1-AP(PYCR2 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human ovary using 17146-1-AP(PYCR2 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue