Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 17146-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#17146-1-AP, RRID:AB_2253344
- Product name
- PYCR2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PYCR2 antibody (Cat. #17146-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Interactomes of SARS-CoV-2 and human coronaviruses reveal host factors potentially affecting pathogenesis.
The upregulation of PYCR2 is associated with aggressive colon cancer progression and a poor prognosis.
Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.
PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.
African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.
Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.
Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.
Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.
Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Kindlin-2 links mechano-environment to proline synthesis and tumor growth.
Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.
A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder.
Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.
Chen Z, Wang C, Feng X, Nie L, Tang M, Zhang H, Xiong Y, Swisher SK, Srivastava M, Chen J
The EMBO journal 2021 Sep 1;40(17):e107776
The EMBO journal 2021 Sep 1;40(17):e107776
The upregulation of PYCR2 is associated with aggressive colon cancer progression and a poor prognosis.
Wang S, Gu L, Huang L, Fang J, Liu Z, Xu Q
Biochemical and biophysical research communications 2021 Oct 1;572:20-26
Biochemical and biophysical research communications 2021 Oct 1;572:20-26
Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.
Jones B, Balasubramaniam M, Lebowitz JJ, Taylor A, Villalta F, Khoshbouei H, Grueter C, Grueter B, Dash C, Pandhare J
Scientific reports 2021 Jan 14;11(1):1422
Scientific reports 2021 Jan 14;11(1):1422
PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.
Cui C, Wang J, Guo L, Wu C
Amino acids 2021 Dec;53(12):1875-1890
Amino acids 2021 Dec;53(12):1875-1890
African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.
Chen S, Zhang X, Nie Y, Li H, Chen W, Lin W, Chen F, Xie Q
Virologica Sinica 2021 Apr;36(2):196-206
Virologica Sinica 2021 Apr;36(2):196-206
Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.
Tran DH, Kesavan R, Rion H, Soflaee MH, Solmonson A, Bezwada D, Vu HS, Cai F, Phillips JA 3rd, DeBerardinis RJ, Hoxhaj G
Nature metabolism 2021 Apr;3(4):571-585
Nature metabolism 2021 Apr;3(4):571-585
Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.
Schwörer S, Berisa M, Violante S, Qin W, Zhu J, Hendrickson RC, Cross JR, Thompson CB
The EMBO journal 2020 Apr 15;39(8):e103334
The EMBO journal 2020 Apr 15;39(8):e103334
Integrated Proteogenomic Characterization of HBV-Related Hepatocellular Carcinoma.
Gao Q, Zhu H, Dong L, Shi W, Chen R, Song Z, Huang C, Li J, Dong X, Zhou Y, Liu Q, Ma L, Wang X, Zhou J, Liu Y, Boja E, Robles AI, Ma W, Wang P, Li Y, Ding L, Wen B, Zhang B, Rodriguez H, Gao D, Zhou H, Fan J
Cell 2019 Oct 3;179(2):561-577.e22
Cell 2019 Oct 3;179(2):561-577.e22
Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606
Kindlin-2 links mechano-environment to proline synthesis and tumor growth.
Guo L, Cui C, Zhang K, Wang J, Wang Y, Lu Y, Chen K, Yuan J, Xiao G, Tang B, Sun Y, Wu C
Nature communications 2019 Feb 19;10(1):845
Nature communications 2019 Feb 19;10(1):845
Oncogenic IDH1 Mutations Promote Enhanced Proline Synthesis through PYCR1 to Support the Maintenance of Mitochondrial Redox Homeostasis.
Hollinshead KER, Munford H, Eales KL, Bardella C, Li C, Escribano-Gonzalez C, Thakker A, Nonnenmacher Y, Kluckova K, Jeeves M, Murren R, Cuozzo F, Ye D, Laurenti G, Zhu W, Hiller K, Hodson DJ, Hua W, Tomlinson IP, Ludwig C, Mao Y, Tennant DA
Cell reports 2018 Mar 20;22(12):3107-3114
Cell reports 2018 Mar 20;22(12):3107-3114
A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder.
Huang YW, Chiang MF, Ho CS, Hung PL, Hsu MH, Lee TH, Chu LJ, Liu H, Tang P, Victor Ng W, Lin DS
Aging and disease 2018 Dec;9(6):1043-1057
Aging and disease 2018 Dec;9(6):1043-1057
Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.
Liu W, Hancock CN, Fischer JW, Harman M, Phang JM
Scientific reports 2015 Nov 24;5:17206
Scientific reports 2015 Nov 24;5:17206
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17146-1-AP(PYCR2 antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using PYCR2 antibody 17146-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human ovary using 17146-1-AP(PYCR2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human ovary using 17146-1-AP(PYCR2 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue