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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-19507 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LEFTY Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-19507 targets Lefty in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples. The PA5-19507 immunogen is synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Lefty. PA5-19507 detects Lefty which has a predicted molecular weight of approximately 44 kDa.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Recombinant Mouse Lefty-1 Protein using Product # PA5-19507, Lefty primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Recombinant Human Lefty-1 Protein at a dilution of 1 µg/mL (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
