Explore
Validate
LearnR32470
antibody from NSJ Bioreagents
Targeting: APOBEC3G
bK150C2.7, CEM15, dJ494G10.1, FLJ12740, MDS019
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R32470 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- APOBEC3G Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific APOBEC3G antibody is suitable for use in Western blot/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- Prior to reconstitution, store at 4oC. After reconstitution, the APOBEC3G antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human A431 and 2) human Jurkat cell lysate with APOBEC3G antibody at 0.5ug/ml. Predicted molecular weight: ~46 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human Jurkat, 2) human HEL, 3) human RT4, 4) rat testis, 5) rat PC-12, 6) mouse testis and 7) mouse RAW264.7 cell lysate with APOBEC3G antibody at 0.5ug/ml. Predicted molecular weight: ~46 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human A431 cells with APOBEC3G antibody (green). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human pancreatic cancer tissue with APOBEC3G antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with APOBEC3G antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human THP-1 cells with APOBEC3G antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=APOBEC3G antibody.
