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antibody from Invitrogen Antibodies
Targeting: APOBEC3G
bK150C2.7, CEM15, dJ494G10.1, FLJ12740, MDS019
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- PA5-78801 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APOBEC3G Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APOBEC3G in A431 whole cell lysate (lane 1), and JURKAT whole cell lysate (lane 2). Sample was incubated with APOBEC3G polyclonal antibody (Product # PA5-78801) at a dilution of 0.5 µg/mL. Signal development was performed using a chemiluminescence (ECL) kit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of APOBEC3G in, Lane 1: human Daudi whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with APOBEC3G Polyclonal Antibody (Product # PA5-78801) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for APOBEC3G at approximately 40 kDa. The expected band size for APOBEC3G is at 46 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of APOBEC3G on paraffin-embedded human tonsil tissue. Sample was incubated with APOBEC3G polyclonal antibody (Product# PA5-78801) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of APOBEC3G on paraffin-embedded human pancreatic cancer tissue. Sample was incubated with APOBEC3G polyclonal antibody (Product# PA5-78801) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of APOBEC3G in THP-1 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with APOBEC3G Polyclonal Antibody (Product # PA5-78801) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
