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antibody from Invitrogen Antibodies
Targeting: APOBEC3G
bK150C2.7, CEM15, dJ494G10.1, FLJ12740, MDS019
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-78801 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- APOBEC3G Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: human Jurkat whole cell lysates, human HEL whole cell lysates, human RT4 whole cell lysates, rat testis tissue lysates, rat PC-12 whole cell lysates, mouse testis tissue lysates, mouse RAW264.7 whole cell lysates. ICC/IF: A431 cell.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of APOBEC3G in A431 cells using APOBEC3G Polyclonal Antibody (Product # PA5-78801). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of APOBEC3G on paraffin-embedded human tonsil tissue. Sample was incubated with APOBEC3G polyclonal antibody (Product# PA5-78801) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of APOBEC3G on paraffin-embedded human pancreatic cancer tissue. Sample was incubated with APOBEC3G polyclonal antibody (Product# PA5-78801) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of APOBEC3G in THP-1 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with APOBEC3G Polyclonal Antibody (Product # PA5-78801) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of APOBEC3G in THP-1 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with APOBEC3G Polyclonal Antibody (Product # PA5-78801) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
