MAB2160-100

antibody from R&D Systems

Targeting: CCR9 CDw199, GPR-9-6, GPR28

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2160-100

Provider

R&D Systems

Product name

Mouse CCR9 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse CCR9 in flow cytometry. Stains mouse CCR9-transfected cells but not irrelevant transfectants.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

Q9WUT7


Isotype

IgG

Antibody clone number

242503

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Gut memories do not fade: epigenetic regulation of lasting gut homing receptor expression in CD4⁺ memory T cells.

Szilagyi BA, Triebus J, Kressler C, de Almeida M, Tierling S, Durek P, Mardahl M, Szilagyi A, Floess S, Huehn J, Syrbe U, Walter J, Polansky JK, Hamann A
Mucosal immunology 2017 Nov;10(6):1443-1454

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Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties.

Yokota-Nakatsuma A, Ohoka Y, Takeuchi H, Song SY, Iwata M
Scientific reports 2016 Nov 29;6:37914

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Retinoic acid primes human dendritic cells to induce gut-homing, IL-10-producing regulatory T cells.

Bakdash G, Vogelpoel LT, van Capel TM, Kapsenberg ML, de Jong EC
Mucosal immunology 2015 Mar;8(2):265-78

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TLR activation excludes circulating naive CD8+ T cells from gut-associated lymphoid organs in mice.

Heidegger S, Kirchner SK, Stephan N, Bohn B, Suhartha N, Hotz C, Anz D, Sandholzer N, Stecher B, Rüssmann H, Endres S, Bourquin C
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5313-20

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E2A proteins promote development of lymphoid-primed multipotent progenitors.

Dias S, Månsson R, Gurbuxani S, Sigvardsson M, Kee BL
Immunity 2008 Aug 15;29(2):217-27

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Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.

Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3724-33

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Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects.

Ueha S, Murai M, Yoneyama H, Kitabatake M, Imai T, Shimaoka T, Yonehara S, Ishikawa S, Matsushima K
Journal of leukocyte biology 2007 Jan;81(1):176-85

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CCR9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CCR9 Monoclonal Antibody (Catalog # MAB2160, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.