PA5-35870

RADX antibody from Invitrogen Antibodies

CXorf57, FLJ10178, FLJ14191

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Other assay

Supportive data in Antibodypedia

Provider product page for PA5-35870

Antibody Data

Product number

PA5-35870

Provider

Invitrogen Antibodies

Product name

CXorf57 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Based on 100% sequence identity, this antibody is predicted to react with Orangutan

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.71 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CXorf57 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXorf57 Polyclonal Antibody (Product # PA5-35870) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CXorf57 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXorf57 Polyclonal Antibody (Product # PA5-35870) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CXorf57 showing staining in the cytoplasm and nucleus of SKNSH cells. SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CXorf57 polyclonal antibody (Product # PA5-35870) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CXorf57 Polyclonal Antibody detects CXorf57 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXorf57 protein stained by CXorf57 Polyclonal Antibody (Product # PA5-35870) diluted at 1:500. Blue: Hoechst 33342 staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CXorf57 showing staining in the cytoplasm and nucleus of SKNSH cells. SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CXorf57 polyclonal antibody (Product # PA5-35870) diluted at 1:500. Blue: Hoechst 33342 staining.