Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-2884 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2884, RRID:AB_10001100
- Product name
- Rabbit Polyclonal RBM14 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM14 Antibody [NB100-2884] - Detection of Human and Mouse CoAA by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), 293T (50ug), Jurkat (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-CoAA antibody NB100-2884 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RBM14 Antibody [NB100-2884] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CoAA antibody used for IP at 6 ug/mg lysate. CoAA was also Immunoprecipitated by a previous lot this antibody. For blotting immunoprecipitated CoAA, NB100-2884 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
