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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PPS003 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal CaM Kinase II Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, rat, bovine, chicken, Xenopus ~50 kDa alpha-CaM Kinase II and the ~60 kDa beta-CaM Kinase II phosphorylated at T305 in Western blots.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Xenopus
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 uL
- Storage
- For long-term storage, = -20degrees C is recommended. Product is stable at = -20degrees C for at least 1 year.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Phospho-CaM Kinase II (T286)by Western Blot. Western blot of 10 μg of rat brain tissue lysate showing specific immunolabeling of alpha- and beta-CaM Kinase II subunits phosphorylated at T305. The labeling by the antibody to CaM Kinase II (T305) is specifically blocked by the T305 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).
