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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PPS002 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-CaM Kinase II (T286) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, rat, bovine, chicken, Xenopus alpha-CaM Kinase II phosphorylated at T286 in Western blots.
- Reactivity
- Human, Mouse, Rat, Xenopus
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
Submitted references Ear2 deletion causes early memory and learning deficits in APP/PS1 mice.
Kummer MP, Hammerschmidt T, Martinez A, Terwel D, Eichele G, Witten A, Figura S, Stoll M, Schwartz S, Pape HC, Schultze JL, Weinshenker D, Heneka MT, Urban I
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jun 25;34(26):8845-54
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jun 25;34(26):8845-54
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Phospho-CaM Kinase II (T286) by Western Blot. Western Blot of rat brain tissue lysate showing specific immunolabeling of the ~50 kDa alpha-CaMKII subunit phosphorylated at T286 and the ~60 kDa beta-CaMKII subunit phosphorylated at T287 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the anti-phospho-CaMKII (T286). The immunolabeling is completely eliminated by treatment with lambda-PPase.
