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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-85747 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CaMKII beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%). Positive Control: mouse brain, rat brain. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.24 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CaMKII beta in various tissue extracts using CaMKII beta polyclonal antibody (Product # PA5-85747) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CaMKII beta was performed by separating 30 µg of human tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CaMKII beta Polyclonal Antibody (Product # PA5-85747) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CaMKII beta Polyclonal Antibody (Product # PA5-85747). Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII beta Polyclonal Antibody (Product # PA5-85747) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CaMKII beta Polyclonal Antibody detects CaMKII beta protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: CaMKII beta protein stained by CaMKII beta Polyclonal Antibody (Product # PA5-85747) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CaMKII beta Polyclonal Antibody detects CAMK2B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CaMKII beta Polyclonal Antibody (Product # PA5-85747) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CaMKII beta Polyclonal Antibody detects CaMKII beta protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: CaMKII beta protein stained by CaMKII beta Polyclonal Antibody (Product # PA5-85747) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
