16-5129-82
antibody from Invitrogen Antibodies
Targeting: LILRB1
CD85, CD85j, ILT2, LIR-1, LIR1, MIR-7, PIR-B, PIRB
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 16-5129-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD85j (ILT2) Monoclonal Antibody (HP-F1), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F. The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells. Applications Reported: This HP-F1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HP-F1 antibody has been tested by flow cytometric analysis of normal human peripheral cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HP-F1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Targeting Ewing sarcoma with activated and GD2-specific chimeric antigen receptor-engineered human NK cells induces upregulation of immune-inhibitory HLA-G.
Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.
Degenerate recognition of MHC class I molecules with Bw4 and Bw6 motifs by a killer cell Ig-like receptor 3DL expressed by macaque NK cells.
A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells.
Kailayangiri S, Altvater B, Spurny C, Jamitzky S, Schelhaas S, Jacobs AH, Wiek C, Roellecke K, Hanenberg H, Hartmann W, Wiendl H, Pankratz S, Meltzer J, Farwick N, Greune L, Fluegge M, Rossig C
Oncoimmunology 2017;6(1):e1250050
Oncoimmunology 2017;6(1):e1250050
Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.
Cheent KS, Jamil KM, Cassidy S, Liu M, Mbiribindi B, Mulder A, Claas FH, Purbhoo MA, Khakoo SI
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 15;110(42):16981-6
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 15;110(42):16981-6
Degenerate recognition of MHC class I molecules with Bw4 and Bw6 motifs by a killer cell Ig-like receptor 3DL expressed by macaque NK cells.
Maloveste SM, Chen D, Gostick E, Vivian JP, Plishka RJ, Iyengar R, Kruthers RL, Buckler-White A, Brooks AG, Rossjohn J, Price DA, Lafont BA
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4338-48
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4338-48
A common inhibitory receptor for major histocompatibility complex class I molecules on human lymphoid and myelomonocytic cells.
Colonna M, Navarro F, Bellón T, Llano M, García P, Samaridis J, Angman L, Cella M, López-Botet M
The Journal of experimental medicine 1997 Dec 1;186(11):1809-18
The Journal of experimental medicine 1997 Dec 1;186(11):1809-18
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were stained with 0.06 µg of Mouse IgG1 kappa Isotype Control (Product # 14-4714-85) (blue histogram) or 0.06 µg of CD85j Monoclonal Antibody, Functional Grade (purple histogram) followed by Anti-IgG1 Monoclonal Antibody, Biotin (Product # 13-4015-82) and Streptavidin, PE (Product # 12-4317-87). Cells in the monocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL