16-5129-82

antibody from Invitrogen Antibodies
Targeting: LILRB1 CD85, CD85j, ILT2, LIR-1, LIR1, MIR-7, PIR-B, PIRB
Provider product page for 16-5129-82

Antibody data

Product number
16-5129-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD85j (ILT2) Monoclonal Antibody (HP-F1), Functional Grade, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F. The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells. Applications Reported: This HP-F1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HP-F1 antibody has been tested by flow cytometric analysis of normal human peripheral cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
HP-F1
Vial size
100 µg
Concentration
1 mg/mL
Storage
4° C
LILRB1 protein structure - 16-5129-82 shown in red.