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LearnH043T03R03
antibody from Invitrogen Antibodies
Targeting: LILRB1
CD85, CD85j, ILT2, LIR-1, LIR1, MIR-7, PIR-B, PIRB
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- H043T03R03 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor™ Red 700, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F. The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells.
- Conjugate
- Near infrared dye
- Antibody clone number
- HP-F1
- Concentration
- 5 µL/Test
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Normal human peripheral blood cells were unstained (left) or stained with CD85j (ILT2) Monoclonal Antibody, NovaFluor Red 700 (Product # H043T03R03) (right). All cells were co-stained with CD11b Monoclonal Antibody, Super Bright 780 (Product # 78-0118-42). Total viable cells in the monocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
- Conjugate
- Near infrared dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Spectral signature for NovaFluor Red 700 collected on a 5-laser Cytek Aurora Full Spectrum flow cytometer using Cytek assay settings. Human peripheral blood mononuclear cells were stained with anti-human CD4 (SK3) and signatures displayed following gating on the lymphocyte population.
- Conjugate
- Near infrared dye
