Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-29529 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p53R2 Recombinant Rabbit Monoclonal Antibody (1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 1
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of p53R2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with p53R2 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29529, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human p53R2 in human squamous cancer with p53R2 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29529, 1:200, formalin-fixed paraffin embedded sections).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human p53R2 in human tonsil with p53R2 Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29529, 1:200, formalin-fixed paraffin embedded sections).