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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09423PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09423PU-N, RRID:AB_2035934
- Product name
- anti RRM2B / P53R2
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.82 mg/mL (by UV absorbance at 280nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4: 293T transfected with ScRNA. Lane 5: 293T tranfected with p53R2 SiRNA The band at 40.7 kDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kDa. The highest band at 44.3 kDa corresponds to recombinant p53R2. Primary antibody was diluted to 1µg/mL and incubated overnight at 4°C. ECL detection was used.
