Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB4348 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TRAM/TICAM2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse TRAM/TICAM2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAM/TICAM2 or rhTRIF/TICAM1 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q8BJQ4
- Isotype
- IgG
- Antibody clone number
- 757706
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse TRAM/TICAM2 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, L-929 mouse fibroblast cell line, and mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TRAM/TICAM2 Monoclonal Antibody (Catalog # MAB4348) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TRAM/TICAM2 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.