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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3673 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3673, RRID:AB_1571733
- Product name
- BMP4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of BMP4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of a putative autocrine bone morphogenetic protein-signaling pathway in human ovarian surface epithelium and ovarian cancer cells.
Bone morphogenic protein 4 produced in endothelial cells by oscillatory shear stress stimulates an inflammatory response.
Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells.
Shepherd TG, Nachtigal MW
Endocrinology 2003 Aug;144(8):3306-14
Endocrinology 2003 Aug;144(8):3306-14
Bone morphogenic protein 4 produced in endothelial cells by oscillatory shear stress stimulates an inflammatory response.
Sorescu GP, Sykes M, Weiss D, Platt MO, Saha A, Hwang J, Boyd N, Boo YC, Vega JD, Taylor WR, Jo H
The Journal of biological chemistry 2003 Aug 15;278(33):31128-35
The Journal of biological chemistry 2003 Aug 15;278(33):31128-35
Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells.
Chadwick K, Wang L, Li L, Menendez P, Murdoch B, Rouleau A, Bhatia M
Blood 2003 Aug 1;102(3):906-15
Blood 2003 Aug 1;102(3):906-15
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BMP4 (arrow) using BMP4 polyclonal antibody (Cat # PAB3673).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BMP4 gene (Lane 2).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MDA-MB-435 cell lysate (35 ug/lane) with BMP4 polyclonal antibody (Cat # PAB3673).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with BMP4 polyclonal antibody (Cat # PAB3673) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
