Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB5020-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse BMP-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects mouse BMP-4 in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P21275
- Isotype
- IgG
- Antibody clone number
- 1128D
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse BMP-4 by Western Blot. Western blot shows Recombinant Mouse BMP-4 (Catalog # 5020-BP) and lysates of mouse embryo tissue. PVDF membrane was probed with 0.25 μg/mL of Rabbit Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB5020) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for BMP-4 at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BMP-4 in Mouse Embryo. BMP-4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rabbit Anti-Mouse BMP-4 Monoclonal Antibody (Catalog # MAB5020) at 2 μg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastrointestinal tract cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.