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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- UM500038 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BMP4 Monoclonal Antibody (UMAB42), UltraMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB42
- Vial size
- 100 µL
- Concentration
- 0.5-1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected pCMV6-ENTRY BTRC (RC219979, Left lane) cDNA for 48 hrs or HEK293T cells untransfected (Right lane) were lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-BTRC.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected pCMV6-ENTRY BTRC (RC219979, Left lane) cDNA for 48 hrs or HEK293T cells untransfected (Right lane) were lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-BTRC.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of 10 different human tissue lysates (10 µg) by using anti-BMP4 monoclonal antibody (clone UMAB42, 1:500)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-BMP4 mouse monoclonal antibody (UM500038, green, 1:100). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human stomach carcinoma using BMP4 clone UMAB42, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500038 requires heat-induced epitope retrieval with Accel pH8.7 at 110°C for 3 min using pressure chamber/cooker. The image shows strong membranous and cytoplasmic staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human small intestine using BMP4 clone UMAB42, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500038 requires heat-induced epitope retrieval with Accel pH8.7 at 110°C for 3 min using pressure chamber/cooker. The image shows strong membranous and cytoplasmic staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human placenta using BMP4 clone UMAB42, mouse monoclonal antibody at 1:100 dilution of mg/mL using Polink2 Broad HRP DAB for detection. UM500038 requires heat-induced epitope retrieval with Accel pH8.7 at 110°C for 3 min using pressure chamber/cooker. The image shows strong membranous and cytoplasmic staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human melanoma using anti-BMP4 mouse monoclonal antibody; an unrelated antibody of same isotype was used as negative control. (Clone UMAB38, dilution 1:50; heat-induced epitope retrieval by 10mM citri
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-BMP4 mouse monoclonal antibody (Clone UMAB38). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-BMP4 (Clone UMAB38) very specifically recognizes BMP4 antigen on OriGene protein microarray chip.
