Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C59678 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C59678, RRID:AB_2144080
- Product name
- MIF Antibody LS-C59678
- Antibody type
- Polyclonal
- Description
- Selective precipitation and salt fractionation followed by daialysis.
- Reactivity
- Human
- Host
- Chicken/Avian
- Isotype
- IgY
- Storage
- Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-Human MIF Polyclonal Antibody - Western Blot. Western blot of IgY fraction of Chicken-anti-Human MIF polyclonal antibody shows the detection of 100 ng of recombinant MIF present in a lysate. Similar detection of MIF will occur when human serum is analyzed. In lane 1 no reaction is observed in the control whereas lane 2 shows a single band at 12.3 kD. A 4-20% gradient gel was used to separate the proteins by SDS-PAGE. The protein was transferred to nitro-cellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Chicken Rabbit IgG [H&L] (code for 1 h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.