20415-1-AP

antibody from Proteintech Group

Targeting: MIF GIF, GLIF

Provider product page for 20415-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [16]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 20415-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20415-1-AP, RRID:AB_10694820
Product name: MIF antibody
Antibody type: Polyclonal
Description: KD/KO validated MIF antibody (Cat. #20415-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: FC, IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MIF protein structure - 20415-1-AP shown in red.

Submitted references Extracellular Vesicles from NSC-34 MN-like Cells Transfected with Mutant SOD1 Modulate Inflammatory Status of Raw 264.7 Macrophages.

Carata E, Muci M, Mariano S, Di Giulio S, Nigro A, Romano A, Panzarini E
Genes 2024 Jun 3;15(6)

DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis.

Wang SY, Zhang SJ, Meng HF, Xu HQ, Guo ZX, Yan JF, Gao JL, Niu LN, Wang SL, Jiao K
Stem cell research & therapy 2024 Apr 23;15(1):113

Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway.

Mao Y, Jiang F, Xu XJ, Zhou LB, Jin R, Zhuang LL, Juan CX, Zhou GP
International journal of biological sciences 2023;19(2):593-609

Exercise prevents fatal stress-induced myocardial injury in obese mice.

Dun Y, Hu Z, You B, Du Y, Zeng L, Zhao Y, Liu Y, Wu S, Cui N, Yang F, Liu S
Frontiers in endocrinology 2023;14:1223423

The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation.

Li S, Liu L, Luo G, Yuan Y, Hu D, Xiao F
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Oct;180:114002

Targeted Knockdown of Macrophage Migration Inhibitory Factor Enhances UVB Irradiation-Induced Apoptosis Via Increasing ROS Generation in Oral Squamous Cell Carcinoma.

Chen T, Chen Q, Li F, Zeng M, Wang B, Huang S, Chen S, Tao Z
Technology in cancer research & treatment 2023 Jan-Dec;22:15330338231163436

Macrophage migration inhibitory factor (MIF) inhibitor iSO-1 promotes staphylococcal protein A-induced osteogenic differentiation by inhibiting NF-κB signaling pathway.

Shi X, Wu Y, Ni H, Li M, Qi B, Xu Y
International immunopharmacology 2023 Feb;115:109600

Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment.

Lu X, Luo Y, Nie X, Zhang B, Wang X, Li R, Liu G, Zhou Q, Liu Z, Fan L, Hotaling JM, Zhang Z, Bo H, Guo J
Nature communications 2023 Dec 20;14(1):8462

Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI.

Li T, Sun H, Li Y, Su L, Jiang J, Liu Y, Jiang N, Huang R, Zhang J, Peng Z
Cell death discovery 2022 Feb 14;8(1):61

TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD).

Zhang X, Gao F, Ai H, Wang S, Song Z, Zheng L, Wang G, Sun Y, Bao Y
Cell proliferation 2021 Apr;54(4):e13015

Proteomic analysis of human periodontal ligament cells under hypoxia.

Li Q, Luo T, Lu W, Yi X, Zhao Z, Liu J
Proteome science 2019;17:3

Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.

Wang SS, Zheng M, Pang X, Zhang M, Yu XH, Wu JB, Gao XL, Wu JS, Yang X, Tang YJ, Tang YL, Liang XH
Molecular carcinogenesis 2019 Oct;58(10):1809-1821

Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.

Liu RM, Sun DN, Jiao YL, Wang P, Zhang J, Wang M, Ma J, Sun M, Gu BL, Chen P, Liu K, Ma H, Gao SG, Ma YF, Qi YJ
Cancer letters 2018 Jan 1;412:289-296

Macrophage migration inhibitory factor promotes vasculogenic mimicry formation induced by hypoxia via CXCR4/AKT/EMT pathway in human glioblastoma cells.

Guo X, Xu S, Gao X, Wang J, Xue H, Chen Z, Zhang J, Guo X, Qian M, Qiu W, Li G
Oncotarget 2017 Oct 6;8(46):80358-80372

A novel strategy of profiling the mechanism of herbal medicines by combining network pharmacology with plasma concentration determination and affinity constant measurement.

Chen L, Lv D, Wang D, Chen X, Zhu Z, Cao Y, Chai Y
Molecular bioSystems 2016 Oct 18;12(11):3347-3356

Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.

Li S, Li X, Wang Y, Yang J, Chen Z, Shan S
BMC microbiology 2014 Feb 7;14:27

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 20415-1-AP(MIF antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human lung using 20415-1-AP(MIF antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The MIF antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human MIF. This antibody recognizes human antigen. The MIF antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 20415-1-AP.pdf

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