Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- AF-289-PB - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal MIF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat MIF in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Targeting the MIF/CXCR7/AKT Signaling Pathway in Castration-Resistant Prostate Cancer.
Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.
An hPSC-Derived Tissue-Resident Macrophage Model Reveals Differential Responses of Macrophages to ZIKV and DENV Infection.
MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells.
Human liver stem cell-derived microvesicles inhibit hepatoma growth in SCID mice by delivering antitumor microRNAs.
Effect of macrophage migration inhibitory factor (MIF) in human placental explants infected with Toxoplasma gondii depends on gestational age.
Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis.
Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells.
Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
Sex dimorphism in wound healing: the roles of sex steroids and macrophage migration inhibitory factor.
Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha.
Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestation.
High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
Rafiei S, Gui B, Wu J, Liu XS, Kibel AS, Jia L
Molecular cancer research : MCR 2019 Jan;17(1):263-276
Molecular cancer research : MCR 2019 Jan;17(1):263-276
Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.
Jalili R, Horecka J, Swartz JR, Davis RW, Persson HHJ
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
An hPSC-Derived Tissue-Resident Macrophage Model Reveals Differential Responses of Macrophages to ZIKV and DENV Infection.
Lang J, Cheng Y, Rolfe A, Hammack C, Vera D, Kyle K, Wang J, Meissner TB, Ren Y, Cowan C, Tang H
Stem cell reports 2018 Aug 14;11(2):348-362
Stem cell reports 2018 Aug 14;11(2):348-362
MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells.
DiCosmo-Ponticello CJ, Hoover D, Coffman FD, Cohen S, Cohen MC
Cytokine 2014 Sep;69(1):47-55
Cytokine 2014 Sep;69(1):47-55
Human liver stem cell-derived microvesicles inhibit hepatoma growth in SCID mice by delivering antitumor microRNAs.
Fonsato V, Collino F, Herrera MB, Cavallari C, Deregibus MC, Cisterna B, Bruno S, Romagnoli R, Salizzoni M, Tetta C, Camussi G
Stem cells (Dayton, Ohio) 2012 Sep;30(9):1985-98
Stem cells (Dayton, Ohio) 2012 Sep;30(9):1985-98
Effect of macrophage migration inhibitory factor (MIF) in human placental explants infected with Toxoplasma gondii depends on gestational age.
de Oliveira Gomes A, de Oliveira Silva DA, Silva NM, de Freitas Barbosa B, Franco PS, Angeloni MB, Fermino ML, Roque-Barreira MC, Bechi N, Paulesu LR, Dos Santos MC, Mineo JR, Ferro EA
The American journal of pathology 2011 Jun;178(6):2792-801
The American journal of pathology 2011 Jun;178(6):2792-801
Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis.
Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S
Proceedings of the National Academy of Sciences of the United States of America 2011 Jul 19;108(29):12048-53
Proceedings of the National Academy of Sciences of the United States of America 2011 Jul 19;108(29):12048-53
Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells.
Ansa-Addo EA, Lange S, Stratton D, Antwi-Baffour S, Cestari I, Ramirez MI, McCrossan MV, Inal JM
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 1;185(9):5236-46
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 1;185(9):5236-46
Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
Meyer-Siegler KL, Cox J, Leng L, Bucala R, Vera PL
Cancer letters 2010 Apr 1;290(1):49-57
Cancer letters 2010 Apr 1;290(1):49-57
Sex dimorphism in wound healing: the roles of sex steroids and macrophage migration inhibitory factor.
Gilliver SC, Ruckshanthi JP, Hardman MJ, Nakayama T, Ashcroft GS
Endocrinology 2008 Nov;149(11):5747-57
Endocrinology 2008 Nov;149(11):5747-57
Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha.
Daryadel A, Grifone RF, Simon HU, Yousefi S
The Journal of biological chemistry 2006 Sep 15;281(37):27653-61
The Journal of biological chemistry 2006 Sep 15;281(37):27653-61
Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestation.
Paulesu L, Cateni C, Romagnoli R, Ietta F, Dantzer V
Biology of reproduction 2005 Apr;72(4):949-53
Biology of reproduction 2005 Apr;72(4):949-53
High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
Chuang CC, Hung CJ, Tsai MC, Yeh TM, Chuang YC
Critical care medicine 2004 Mar;32(3):734-9
Critical care medicine 2004 Mar;32(3):734-9
High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor?
Chuang CC, Hung CJ, Tsai MC, Yeh TM, Chuang YC
Critical care medicine 2004 Mar;32(3):734-9
Critical care medicine 2004 Mar;32(3):734-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human MIF by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-289-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse and Rat MIF by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-289-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MIF at approximately 12-14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.