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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF1978 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal MIF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse MIF in direct ELISAs and human, mouse and rat MIF in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Efficacy of Astaxanthin for the Treatment of Atopic Dermatitis in a Murine Model.
Yoshihisa Y, Andoh T, Matsunaga K, Rehman MU, Maoka T, Shimizu T
PloS one 2016;11(3):e0152288
PloS one 2016;11(3):e0152288
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat MIF by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line, J774A.1 mouse reticulum cell sarcoma macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1978) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
