36-7401

antibody from Invitrogen Antibodies

Targeting: MIF GIF, GLIF

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

36-7401

Provider

Invitrogen Antibodies

Product name

Anti-MIF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

36-7401 detects MIF from human, mouse and rat samples. 36-7401 has been successfully used in Western blot, immunohistochemical, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 12 kDa protein representing MIF. Immunofluorescent staining in U2OS cells and Immunohistochemical staining of MIF in rat epididymis tissue with 36-7401. yields a staining pattern consistent with MIF in nucleus and cytosol.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

Lot Dependent

Storage

-20°C

References

Submitted references

Nicotinamide-functionalized multiwalled carbon nanotubes increase insulin production in pancreatic beta cells via MIF pathway.

Ilie I, Ilie R, Mocan T, Tabaran F, Iancu C, Mocan L
International journal of nanomedicine 2013;8:3345-53

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Macrophage migration inhibitory factor acts as a neurotrophin in the developing inner ear.

Bank LM, Bianchi LM, Ebisu F, Lerman-Sinkoff D, Smiley EC, Shen YC, Ramamurthy P, Thompson DL, Roth TM, Beck CR, Flynn M, Teller RS, Feng L, Llewellyn GN, Holmes B, Sharples C, Coutinho-Budd J, Linn SA, Chervenak AP, Dolan DF, Benson J, Kanicki A, Martin CA, Altschuler R, Koch AE, Jewett EM, Germiller JA, Barald KF
Development (Cambridge, England) 2012 Dec;139(24):4666-74

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The role of macrophage migration inhibitory factor in ocular surface disease pathogenesis after chemical burn in the murine eye.

Oh SY, Choi JS, Kim EJ, Chuck RS, Park CY
Molecular vision 2010 Nov 17;16:2402-11

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Ribosomal protein S19 interacts with macrophage migration inhibitory factor and attenuates its pro-inflammatory function.

Filip AM, Klug J, Cayli S, Fröhlich S, Henke T, Lacher P, Eickhoff R, Bulau P, Linder M, Carlsson-Skwirut C, Leng L, Bucala R, Kraemer S, Bernhagen J, Meinhardt A
The Journal of biological chemistry 2009 Mar 20;284(12):7977-85

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MIF in U2OS cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a MIF mouse monoclonal antibody (Product # 36-7401) at a dilution of 1:100 in blocking buffer for 1 hour at room temperature, and then incubated with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® Plus 488 conjugate (Product # A32731) at a dilution of 1:500 for at least 30 minutes at room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of rat epididymis tissue using Rb anti-MIF (Product # 36-7401).