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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- MAB19781 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat MIF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse MIF in ELISA and Western Blot.
- Reactivity
- Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P34884- Isotype
- IgM
- Antibody clone number
- 811409
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse MIF by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB19781) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Rat MIF by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB19781) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of MIF in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse/Rat MIF Monoclonal Antibody (Catalog # MAB19781, filled histogram) or rat IgM isotype control antibody, followed by Allophycocyanin-conjugated anti-rat IgM. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
