Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 524001 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#524001, RRID:AB_2562323
- Product name
- Purified anti-mouse/rat/human MIF
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant mouse MIF (AA: Pro2-Ala115) expressed in E. coli (Cat# 599504).
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antibody clone number
- Poly5240
- Vial size
- 200 µl
- Storage
- -20°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot analysis of cell lysates from HeLa (lane 1), U937 (lane 2), Raw264.7 (lane 3), and rat brain (lane 4). Purified anti-MIF antibody (clone Poly5240) was used and was followed by HRP anti-goat IgG antibody. GAPDH (clone Poly6314) was used as a loading control.
- Conjugate
- Unconjugated