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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB1071 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1071, RRID:AB_1576961
- Product name
- MIF monoclonal antibody, clone 4E4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant MIF.
- Isotype
- IgG
- Antibody clone number
- 4E4
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references MIF rediscovered: cytokine, pituitary hormone, and glucocorticoid-induced regulator of the immune response.
Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF).
Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor.
Bucala R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1996 Dec;10(14):1607-13
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1996 Dec;10(14):1607-13
Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF).
Bernhagen J, Mitchell RA, Calandra T, Voelter W, Cerami A, Bucala R
Biochemistry 1994 Nov 29;33(47):14144-55
Biochemistry 1994 Nov 29;33(47):14144-55
Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor.
Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC, David JR
Proceedings of the National Academy of Sciences of the United States of America 1989 Oct;86(19):7522-6
Proceedings of the National Academy of Sciences of the United States of America 1989 Oct;86(19):7522-6
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- Abnova Corporation (provider)
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- Experimental details
- Western blot analysis of HL-60 extract was resolved by SDS - PAGE , transferred to PVDF membrane and probed with MIF monoclonal antibody , clone 4E4 (1 : 1000) (Cat # MAB1071) . Protein was visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
