Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-32192 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-32192, RRID:AB_2144078
- Product name
- Rabbit Polyclonal MIF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MIF Antibody [NBP1-32192] - Sample (30 ug of whole cell lysate)A: Molt-4 15% SDS PAGE, antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MIF Antibody [NBP1-32192] - ELISA detection of MIF [N1C3] capture at a concentration of 5 ug/mL and MIF C-term antibody for detection at a concentration of 1.5 ug/mL.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - : Paraffin-embedded rat brain. MIF antibody [N1C3] diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - Paraffin-embedded mouse brain. MIF antibody [N1C3] diluted at 1:500.