PA5-18383

antibody from Invitrogen Antibodies

Targeting: MIF GIF, GLIF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18383

Provider

Invitrogen Antibodies

Product name

MIF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse and rat based on sequence homology.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MIF by a MIF monoclonal antibody (Product # PA5-18383) at a concentration of 0.01 µg/mL. Human Thymus lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of Human Thymus lysate using Product # PA5-18383 at a concentration of 0.01 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MIF by a MIF monoclonal antibody (Product # PA5-18383) at a concentration of 0.01 µg/mL. Daudi (A) and Jurkat (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MIF in Human Thymus using a MIF monoclonal antibody (Product #PA5-18383) at 5 µg/mL. The Human Thymus tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA of MIF using two MIF antibodies. The reporter antibody (Product # PA5-18383) was used at a concentration of 1.5 µg/ml and the reporter antibody was used at a concentration of 5 µg/ml.