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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- LS-C19086 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C19086, RRID:AB_2301988
- Product name
- SLIT2 Antibody LS-C19086
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Submitted references Slit2 overexpression results in increased microvessel density and lesion size in mice with induced endometriosis.
Guo SW, Zheng Y, Lu Y, Liu X, Geng JG
Reproductive sciences (Thousand Oaks, Calif.) 2013 Mar;20(3):285-98
Reproductive sciences (Thousand Oaks, Calif.) 2013 Mar;20(3):285-98
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-SLIT-2 Antibody - Western Blot. Western blot of Affinity Purified anti-SLIT-2 antibody shows detection of a band at ~165 kD (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of Rabbit anti SLIT-2 Antibody in human breast carcinoma. Tissue: Human prostate. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker, EDTA pH 9.5 Pressure Cooker. Primary antibody: SLIT2 at 2ug/ml for 1.5 hour @ room Temp. Secondary Ab: MACH 1 HRP POLYMER at 1:50 for 45” RT.
