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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Other assay [2]
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- Product number
- PA5-31133 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLIT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, SK-N-AS, mouse brain, rat brain, SLIT2-transfected 293T. Predicted reactivity: Mouse (98%), Rat (95%), Zebrafish (88%), Xenopus laevis (93%), Chicken (97%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.17 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Slit2 Modulates the Inflammatory Phenotype of Orbit-Infiltrating Fibrocytes in Graves' Disease.
Fernando R, Grisolia ABD, Lu Y, Atkins S, Smith TJ
Journal of immunology (Baltimore, Md. : 1950) 2018 Jun 15;200(12):3942-3949
Journal of immunology (Baltimore, Md. : 1950) 2018 Jun 15;200(12):3942-3949
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- SLIT2 Polyclonal Antibody immunoprecipitates SLIT2 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 µg whole cell lysate/extract of SLIT2 protein expressing Raji cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of SLIT2 by 3 µg of SLIT2 Polyclonal Antibody (Product # PA5-31133) 5% SDS-PAGE The immunoprecipitated SLIT2 protein was detected by SLIT2 Polyclonal Antibody (Product # PA5-31133) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
