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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [9]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- PA5-31133 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLIT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, SK-N-AS, mouse brain, rat brain, SLIT2-transfected 293T.
- Concentration
- 1.17 mg/mL
Submitted references Slit2 Modulates the Inflammatory Phenotype of Orbit-Infiltrating Fibrocytes in Graves' Disease.
Fernando R, Grisolia ABD, Lu Y, Atkins S, Smith TJ
Journal of immunology (Baltimore, Md. : 1950) 2018 Jun 15;200(12):3942-3949
Journal of immunology (Baltimore, Md. : 1950) 2018 Jun 15;200(12):3942-3949
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLIT2 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg Jurkat whole cell lysate and D) 30 µg Raji whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SLIT2 polyclonal antibody (Product # PA5-31133) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLIT2 using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a SLIT2 polyclonal antibody (Product # PA5-31133) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLIT2 in Various whole cell extracts (30 µg). Samples were separated by 5% SDS-PAGE and the membrane was probed with SLIT2 Polyclonal antibody (Product # PA5-31133) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SLIT2 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SLIT2 Polyclonal Antibody (Product # PA5-31133) at a dilution of 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SLIT2 Polyclonal Antibody (Product # PA5-31133). Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 Polyclonal Antibody (Product # PA5-31133) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SLIT2 Polyclonal Antibody detects SLIT2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 Polyclonal Antibody (Product # PA5-31133) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLIT2 was performed by separating 30 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SLIT2 Polyclonal Antibody (Product # PA5-31133) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SLIT2 Polyclonal Antibody (Product # PA5-31133). Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 Polyclonal Antibody (Product # PA5-31133) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SLIT2 was performed by separating 50 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SLIT2 Polyclonal Antibody (Product # PA5-31133) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SLIT2 was performed in paraffin-embedded mouse fetal brain tissue using Green: NAGLU Polyclonal Antibody (Product # PA5-28261) at a dilution of 1:200. Blue: Nuclear staining with Hoechst 33342. The signal was developed using a goat anti-rabbit IgG antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SLIT2 Polyclonal Antibody detects SLIT2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SLIT2 Polyclonal Antibody (Product # PA5-31133) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SLIT2 Polyclonal Antibody detects SLIT2 protein at cytosol and membrane on gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. SLIT2 Polyclonal Antibody (Product # PA5-31133) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SLIT2 Polyclonal Antibody immunoprecipitates SLIT2 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : 30 µg whole cell lysate/extract of SLIT2 protein expressing Raji cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of SLIT2 by 3 µg of SLIT2 Polyclonal Antibody (Product # PA5-31133) 5% SDS-PAGE The immunoprecipitated SLIT2 protein was detected by SLIT2 Polyclonal Antibody (Product # PA5-31133) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
