AP09265PU-N

antibody from Acris Antibodies GmbH

Targeting: SLIT2 SLIL3, Slit-2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09265PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09265PU-N, RRID:AB_2035960

Product name

anti SLIT2 (484-500)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding aa 484-500 of Human SLIT-2 protein.

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian, Xenopus

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity Purified anti-SLIT-2 antibody shows detection of a band at ~165 kDa (lane 1) corresponding to SLIT-2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDSPAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.