PA5-89258

antibody from Invitrogen Antibodies

Targeting: CYP51A1 CP51, CYP51, CYPL1, LDM, P450-14DM, P450L1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89258

Provider

Invitrogen Antibodies

Product name

CYP51A1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: WLLPGWLPLP SFRRRDRAHR EIKDIFYKAI QKRRQSQEKI DDILQTLLDA TYKDGRPLTD DEVAGMLIGL LLAGQHTSST TSAWMGFFLA RDKTLQKKCY LEQKTVCGEN LPPLTYDQLK DLNLLDRCIK ETLRLRPPIM IMMRMARTPQ TVAGYTIPPG HQVCVSPTVN QRLKDS Positive Samples: Cellular Location: Endoplasmic reticulum membrane, Microsome membrane

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.34 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CYP51A1 was performed in MCF-7 cells using CYP51A1 Polyclonal Antibody (Product # PA5-89258).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CYP51A1 in L929 cells. Samples were incubated with CYP51A1 Polyclonal antibody (Product # PA5-89258) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CYP51A1 in paraffin-embedded human thyroid cancer. Samples were incubated with CYP51A1 Polyclonal antibody (Product # PA5-89258) using a dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CYP51A1 in paraffin-embedded mouse kidney. Samples were incubated with CYP51A1 Polyclonal antibody (Product # PA5-89258) using a dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CYP51A1 in paraffin-embedded rat kidney. Samples were incubated with CYP51A1 Polyclonal antibody (Product # PA5-89258) using a dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.