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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- PA5-109207 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AMACR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Human kidney tissue, mouse liver tissue lysate, Siha, SW480, HepG2, human liver tissue, human colon cancer tissue, PC-3M.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AMACR in Lane 1: Mouse liver tissue, Lane 2: SiHa, Lane 3: SW480, Lane 4: Human kidney tissue. Samples were incubated with AMACR polyclonal antibody (Product # PA5-109207), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AMACR in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with AMACR polyclonal antibody (Product # PA5-109207), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of AMACR in paraffin-embedded human liver tissue. Samples were incubated with AMACR polyclonal antibody (Product # PA5-109207), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of AMACR in paraffin-embedded human kidney tissue. Samples were incubated with AMACR polyclonal antibody (Product # PA5-109207), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of AMACR in paraffin-embedded human colon cancer tissue. Samples were incubated with AMACR polyclonal antibody (Product # PA5-109207), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of AMACR in PC-3M cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with AMACR polyclonal antibody (Product # PA5-109207) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.
