Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF7508 - Provider product page
- Provider
- R&D Systems
- Product name
- Human alpha-Methylacyl-CoA Racemase/AMACR Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human alpha-Methylacyl-CoA Racemase/AMACR in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9UHK6
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma.
Wang J, He G, Yang Q, Bai L, Jian B, Li Q, Li Z
Oncology letters 2018 Jun;15(6):9009-9016
Oncology letters 2018 Jun;15(6):9009-9016
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human alpha-Methylacyl-CoA Racemase/AMACR by Western Blot. Western blot shows lysates of human kidney tissue, human liver tissue, and human prostate tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human alpha-Methylacyl-CoA Racemase/AMACR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7508) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha-Methylacyl-CoA Racemase/AMACR at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- alpha-Methylacyl-CoA Racemase/AMACR in Human Prostate Gland. alpha-Methylacyl-CoA Racemase/AMACR was detected in immersion fixed paraffin-embedded sections of human prostate gland using Sheep Anti-Human alpha-Methylacyl-CoA Racemase/AMACR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7508) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.