ARP59971_P050

antibody from Aviva Systems Biology

Targeting: ADSL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ARP59971_P050

Provider

Aviva Systems Biology

Proper citation

Aviva Systems Biology Cat#ARP59971_P050, RRID:AB_10870241

Product name

ADSL antibody - middle region (ARP59971_P050)

Antibody type

Polyclonal

Antigen

The immunogen is a synthetic peptide directed towards the middle region of human ADSL

Description

Affinity Purified

Reactivity

Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Horse, Porcine, Rabbit, Sheep

Host

Rabbit

Vial size

100 μl

Concentration

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Sample Type: 1. Hamster CHO K1 cells (20ug) 2. HeLa skin cells(100ug) 3. HEK273 cells (100ug) 4. HepG2 cells (100ug) 5. purified human ADSL protein (40ng) Primary Dilution: 1:2000 Secondary Antibody: Clean-Blot IP detection Reagent and Kit Secondary Dilution: 1:2000 Image Submitted By: V. Skopova, M. Zikanova Institute of Inherited Metabolic Disorders, Charles University in Prague and General Hopspital in Prague

Sample type

human:HEK273,HepG2, Hela, skin fibroblasts. Hamster:CHO

Primary Ab dilution

1:2000

Protocol

Protocol

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

WB Suggested Anti-ADSL Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:62500, Positive Control: Human Muscle

Sample type

human:HEK273,HepG2, Hela, skin fibroblasts. Hamster:CHO

Primary Ab dilution

0.2-1 µg/mL

Protocol

Protocol