NBP2-31099-0.1mg
antibody from Novus Biologicals
Targeting: AR
AIS, DHTR, HUMARA, NR3C4, SBMA, SMAX1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NBP2-31099-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Androgen R/NR3C4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. In IGF-1 stimulated LNCaP cells (passage number 38), a ~110 kDa band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Androgen R/NR3C4 [p Ser210, p Ser213] Antibody (156C135.2) - Azide Free [NBP2-31099] - LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 min prior to treatment with 100 ng/ml IGF-1 for 4 h. (See Lin, H-K., Y-C Hu, L. Yang, S. Altuwaijri. 2003 Fig. 2)