Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX100056 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100056, RRID:AB_1240448
- Product name
- Androgen Receptor antibody [N1], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: U87-MG 7.5% SDS PAGE GTX100056 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor antibody [N1], N-term (GTX100056) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Androgen Receptor antibody [N1], N-term detects Androgen Receptor protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Androgen Receptor protein stained by Androgen Receptor antibody [N1], N-term (GTX100056) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
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- Experimental details
- Androgen Receptor antibody [N1], N-term detects Androgen Receptor protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Androgen Receptor protein stained by Androgen Receptor antibody [N1], N-term (GTX100056) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Androgen Receptor antibody [N1], N-term detects Androgen Receptor protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Androgen Receptor antibody [N1], N-term (GTX100056) dilution: 1:500.