R34036

antibody from NSJ Bioreagents

Targeting: AR AIS, DHTR, HUMARA, NR3C4, SBMA, SMAX1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

R34036

Provider

NSJ Bioreagents

Product name

Androgen Receptor Antibody

Antibody type

Polyclonal

Description

This highly specific Androgen Receptor antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Immunocytochemistry/ELISA/ChImmunoprecipitation applications with human samples.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Storage

Aliquot and store the Androgen receptor antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human brain lysate with Androgen receptor antibody at 0.3ug/ml. Predicted molecular weight ~99kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human brain lysate with Androgen receptor antibody at 0.3ug/ml. Predicted molecular weight ~99kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human A) cerebellum and B) pancreas (negative control) tissue lysate with Androgen receptor antibody at 0.3ug/ml. Predicted molecular weight ~99 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of fixed and permeabilized human MCF7 cells with Androgen receptor antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC staining of fixed and permeabilized human U-2 OS cells with Androgen receptor antibody (green) at 10ug/ml and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human prostate with Androgen receptor antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human prostate with Androgen receptor antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, AP-staining.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ChIP of 2ug Androgen receptor antibody with 1ug DHT-treated HEC50 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring FKBP5 enrichment.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ChIP of 2ug Androgen receptor antibody with 1ug DHT-treated HEC50 chromatin using the Chromatrap spin column sonication kit (Protein G) measuring FKBP5 enrichment.