M00542-2

antibody from Boster Biological Technology

Targeting: AR AIS, DHTR, HUMARA, NR3C4, SBMA, SMAX1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00542-2

Provider

Boster Biological Technology

Product name

Anti-Androgen Receptor AR Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for ANDROGEN RECEPTOR/AR detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat ANDROGEN RECEPTOR/AR information: Molecular Weight: 98989 MW; Subcellular Localization: Nucleus. Cytoplasm. Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of GNB2L1; Tissue Specificity: Isoform 2 is mainly expressed in heart and skeletal muscle.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

CDF-1

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Sirtuin 6 is a key contributor to gender differences in acute kidney injury.

Miao J, Huang J, Liang Y, Zhang Y, Li J, Meng P, Shen W, Li X, Wu Q, Wang X, Niu H, Tang Y, Zhou S, Zhou L
Cell death discovery 2023 Apr 25;9(1):134

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Explore the mechanism and substance basis of Mahuang FuziXixin Decoction for the treatment of lung cancer based on network pharmacology and molecular docking.

Zhang W, Tian W, Wang Y, Jin X, Guo H, Wang Y, Tang Y, Yao X
Computers in biology and medicine 2022 Dec;151(Pt A):106293

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Oral exposure to low-dose bisphenol A induces hyperplasia of dorsolateral prostate and upregulates EGFR expression in adult Sprague-Dawley rats.

Wu S, Huang D, Su X, Yan H, Wu J, Sun Z
Toxicology and industrial health 2019 Oct;35(10):647-659

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Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone.

Liu X, Cheng YI, Pan QI, Hu W, Xu LI, Meng X, Wu J, Xie C, Yan H, Sun Z
Experimental and therapeutic medicine 2016 Apr;11(4):1361-1366

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Possible mechanism of benign prostatic hyperplasia induced by androgen-estrogen ratios in castrated rats.

Xiang-Yun L, Ying-Wen X, Chen-Jing X, Jiu-Jiu W, Qi P, Bo G, Zu-Yue S
Indian journal of pharmacology 2010 Oct;42(5):312-7

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human prostate, using Androgen Receptor Antibody(M00542-2)AR was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AR Antibody (M00542-2)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image