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LearnA00542
antibody from Boster Biological Technology
Targeting: AR
AIS, DHTR, HUMARA, NR3C4, SBMA, SMAX1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A00542 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Androgen Receptor/AR Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Androgen Receptor/AR detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references JMJD2A promotes the development of castration-resistant prostate cancer by activating androgen receptor enhancer and inhibiting the cGAS-STING pathway.
Sirtuin 6 is a key contributor to gender differences in acute kidney injury.
Hormonal Regulation of Testicular Development in the Finless Porpoise Neophocaena asiaeorientalis sunameri: Preliminary Evidence from Testicular Histology and Immunohistochemistry.
Oral exposure of low-dose bisphenol A promotes proliferation of dorsolateral prostate and induces epithelial-mesenchymal transition in aged rats.
Androgen Receptor Is Inactivated and Degraded in Bladder Cancer Cells by Phenyl Glucosamine via miR-449a Restoration.
Elevated expression levels of androgen receptors and matrix metalloproteinase-2 and -9 in 30 cases of hepatocellular carcinoma compared with adjacent tissues as predictors of cancer invasion and staging.
Effects of extract of Buddleja officinalis eye drops on androgen receptors of lacrimal gland cells of castrated rats with dry eye.
Cai X, Yu X, Tang T, Xu Y, Wu T
Molecular carcinogenesis 2024 Sep;63(9):1682-1696
Molecular carcinogenesis 2024 Sep;63(9):1682-1696
Sirtuin 6 is a key contributor to gender differences in acute kidney injury.
Miao J, Huang J, Liang Y, Zhang Y, Li J, Meng P, Shen W, Li X, Wu Q, Wang X, Niu H, Tang Y, Zhou S, Zhou L
Cell death discovery 2023 Apr 25;9(1):134
Cell death discovery 2023 Apr 25;9(1):134
Hormonal Regulation of Testicular Development in the Finless Porpoise Neophocaena asiaeorientalis sunameri: Preliminary Evidence from Testicular Histology and Immunohistochemistry.
Xiao Y, Nabi G, Hao Y, Wang D
Zoological studies 2018;57:e41
Zoological studies 2018;57:e41
Oral exposure of low-dose bisphenol A promotes proliferation of dorsolateral prostate and induces epithelial-mesenchymal transition in aged rats.
Huang DY, Zheng CC, Pan Q, Wu SS, Su X, Li L, Wu JH, Sun ZY
Scientific reports 2018 Jan 11;8(1):490
Scientific reports 2018 Jan 11;8(1):490
Androgen Receptor Is Inactivated and Degraded in Bladder Cancer Cells by Phenyl Glucosamine via miR-449a Restoration.
Guo J, Hu J, Cao R, Chen Q, Li K
Medical science monitor : international medical journal of experimental and clinical research 2018 Apr 16;24:2294-2301
Medical science monitor : international medical journal of experimental and clinical research 2018 Apr 16;24:2294-2301
Elevated expression levels of androgen receptors and matrix metalloproteinase-2 and -9 in 30 cases of hepatocellular carcinoma compared with adjacent tissues as predictors of cancer invasion and staging.
Zhang Y, Shen Y, Cao B, Yan A, Ji H
Experimental and therapeutic medicine 2015 Mar;9(3):905-908
Experimental and therapeutic medicine 2015 Mar;9(3):905-908
Effects of extract of Buddleja officinalis eye drops on androgen receptors of lacrimal gland cells of castrated rats with dry eye.
Peng QH, Yao XL, Wu QL, Tan HY, Zhang JR
International journal of ophthalmology 2010;3(1):43-8
International journal of ophthalmology 2010;3(1):43-8
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Androgen Receptor/AR using anti-Androgen Receptor/AR antibody (A00542). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Androgen Receptor/AR antigen affinity purified polyclonal antibody (Catalog # A00542) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Androgen Receptor/AR at approximately 120KD. The expected band size for Androgen Receptor/AR is at 120KD.
- Additional image
