RQ5958

antibody from NSJ Bioreagents

Targeting: AR AIS, DHTR, HUMARA, NR3C4, SBMA, SMAX1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5958

Provider

NSJ Bioreagents

Product name

Androgen Receptor Antibody

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the AR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) K562, 2) U-2 OS, 3) HEK293, 4) PC-3, 5) 22RV1, 6) HepG2 and 7) Caco-2 lysate with AR antibody. Predicted molecular weight ~99 kDa, observed at up to ~120 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human T-47D cells with AR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A549 cells with AR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AR antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human RAW264.7 cells with AR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AR antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human C6 cells with AR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AR antibody.