Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- RQ5958 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- AR Antibody / Androgen Receptor
- Antibody type
- Polyclonal
- Description
- This highly specific AR antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the AR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) K562, 2) U-2 OS, 3) HEK293, 4) PC-3, 5) 22RV1, 6) HepG2 and 7) Caco-2 lysate with AR antibody. Predicted molecular weight ~99 kDa, observed at up to ~120 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human T-47D cells with AR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A549 cells with AR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AR antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human RAW264.7 cells with AR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AR antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human C6 cells with AR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AR antibody.
